Genetic analysis of subunits of two major storage proteins (.BETA.-conglycinin and glycinin) in soybean seeds.
نویسندگان
چکیده
منابع مشابه
beta-Conglycinin and glycinin in high-protein soybean seeds.
The agronomic performance and storage proteins of high seed protein lines of soybeans [Glycine max L. (Merr.)] were investigated to determine if the two major storage proteins, beta-conglycinin and glycinin, contribute to the increased protein content of high seed protein lines. Subunits of these two major storage proteins were estimated by scanning SDS-PAGE gels by scanning densitometry. The r...
متن کاملIdentification of glycinin and beta-conglycinin subunits that contribute to the increased protein content of high-protein soybean lines.
Seed protein concentration of commercial soybean cultivars calculated on a dry weight basis ranges from approximately 37 to 42% depending on genotype and location. A concerted research effort is ongoing to further increase protein concentration. Several soybean plant introductions (PI) are known to contain greater than 50% protein. These PIs are exploited by breeders to incorporate the high-pro...
متن کاملDifferential Proteolysis of Glycinin and beta-Conglycinin Polypeptides during Soybean Germination and Seedling Growth.
The degradation of the major seed storage globulins of the soybean (Glycine max [L.] Merrill) was examined during the first 12 days of germination and seedling growth. The appearance of glycinin and beta-conglycinin degradation products was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cotyledon extracts followed by electroblotting to nitrocellulose and immunostaining...
متن کاملCosuppression of the alpha subunits of beta-conglycinin in transgenic soybean seeds induces the formation of endoplasmic reticulum-derived protein bodies.
The expression of the alpha and alpha' subunits of beta-conglycinin was suppressed by sequence-mediated gene silencing in transgenic soybean seed. The resulting seeds had similar total oil and protein content and ratio compared with the parent line. The decrease in beta-conglycinin protein was apparently compensated by an increased accumulation of glycinin. In addition, proglycinin, the precurs...
متن کاملAssessing Glycinin (11S) and b-Conglycinin (7S) Fractions of Soybean Storage Protein by Near-Infrared Spectroscopy
Soybean breeding programs underway today are addressing the goal of improving the protein profile to benefit the human diet as well as that of livestock. Glycinin, a globulin storage protein of the meal and designated as the 11S size fraction by ultracentrifugation, is desirable because of its relative abundance of sulfur-containing amino acids, such as methionine and cysteine. The current stud...
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ژورنال
عنوان ژورنال: Japanese Journal of Breeding
سال: 1986
ISSN: 0536-3683,2185-291X
DOI: 10.1270/jsbbs1951.36.390